Species | Human |
Individual | Patient |
Cell type | Cancer cell |
Cancer type | Prostate |
Age | 56 |
Sex | Male |
TNM Status | T3N0M0 |
Status at the analysis timepoint | Non-metastatic |
Moment of samples collection | Preoperative |
Clinical material | Tumor draining vein blood |
Collection | EDTA tubes |
Fixative/storage | 4% PFA/-80̊ C |
Protocol | Pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31 |
Acquisition conditions | Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW |
Biomarkers | Pan-keratin (K), vimentin (V) |
Phenotype | K (+) / V (+) / DAPI (+) / α-SMA (-) / CD29 (-) / CD45/CD31 (-) |
Clusterization status | Single |
Size | Large (>12 µm) |
Shape | Circle |
Elongation | No |
Protrusions | No |
Micronucleus | No |
Interactions with normal cells | No |
Interactions with | No |