Atlas ID 101

Cell
Category Description
General information
Species Human
Individual Patient
Cell type Fibroblast
Cancer type Breast
Clinical information
Age 57
Sex Female
TNM Status -
Status at the analysis timepoint Non-metastatic
Moment of samples collection Preoperative
Material preparation
Clinical material Peripheral blood
Collection EDTA tubes
Fixative/storage 4% PFA/-80˚C
Protocol Pan-keratin (K) / vimentin (V) / DAPI / α-SMA / CD29 / CD45/CD31
Acquisition conditions Objective 40x, low flow, lasers/power: 405 nm/100 mW, 488 nm /100mW, 647 nm /150 mW
Cell characteristics
Biomarkers α-SMA, vimentin (V)
Phenotype K (-) / V (+) / DAPI (+) / α-SMA (+) / CD29 (-) / CD45/CD31 (-)
Clusterization status Single
Size Large (>12 µm)
Shape Circle
Elongation No
Protrusions No
Micronucleus No
Interactions with normal cells No
Interactions with No